Stichting het Nederlands Kanker Instituut - Antoni van Leeuwenhoek ziekenhuis (The Netherlands Cancer Institute).
AB1 CHROMATOGRAM VIEWER GATC CODE
AB1 CHROMATOGRAM VIEWER GATC SOFTWARE
If you use this software for data analysis in a publication, please cite.The TIDE software is being provided as a free web service for research, educational, instructional and non-commercial purposes only.The output of TIDE is a comprehensive profile of all insertions and deletions (indels) in the edited sample. The input to TIDE is Sanger sequencing data. Based on the quantitative sequence trace data from two standard capillary sequencing reactions the TIDE software quantifies the editing efficacy and identifies the predominant types of insertions and deletions (indels) in the DNA of a targeted cell pool. TIDE provides rapid and reliable assessment of genome editing experiments of a target locus. Reference (please cite!): Brinkman et al, Nucl.Drawbacks: The method will not capture megabase long deletions that can originate by CRISPR/Cas9 induced DSB.
What it needs: Standard capillary sequencing reactions.For templated CRISPR/Cas9 experiments, use the TIDER web tool. If you have a single sample, go to the original TIDE site. When to use: Quantification of small indels for multiple samples.What it does: Estimates the spectrum and frequency of small insertions and deletions (indels) generated in a pool of cells by genome editing tools such as CRISPR/Cas9, TALENs and ZFNs.
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